Dataset · 2021

Phelps, Hildebrand & Graham — Drosophila ventral nerve cord

Motor control circuits in the adult fly ventral nerve cord, and the dataset that introduced GridTape automated section collection.

SpeciesDrosophila melanogaster (female)
RegionVentral nerve cord
Cells507 limb motor neurons reconstructed
ModalityGridTape automated TEM
AccessOpen
StatusReleased

GridTape automates the most failure-prone step in serial-section EM: collecting sections onto a substrate without losing or folding them. This dataset used it to image the adult female Drosophila ventral nerve cord and reconstruct 507 limb motor neurons.

What it is good for. The methods half of Unit 03: it is the clearest illustration of why section collection, not imaging, is the throughput bottleneck.

What it does not support. Whole-VNC connectivity — this reconstruction targets motor neurons rather than the full circuit.

Where these numbers come from. Phelps, Hildebrand, Graham et al. (2021), Cell 10.1016/j.cell.2020.12.013; BossDB. DOI 10.1016/j.cell.2020.12.013.

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