Lee et al. — mouse visual cortex functional network

Lee and colleagues combined in vivo calcium imaging with EM reconstruction to test whether functionally similar pyramidal neurons are preferentially connected. They are — a result that MICrONS later revisited at scale.

What it is good for. A compact example of a hypothesis stated before the data were collected, with a null model, in a volume small enough to reason about.

What it does not support. Strong effect-size claims: the number of connected pairs recovered is small, and the confidence intervals are correspondingly wide.