Dataset · 2020

Hemibrain — Drosophila central brain

A densely reconstructed half of the Drosophila central brain, imaged by FIB-SEM at isotropic resolution and the dataset on which most fly circuit analysis before FlyWire was built.

SpeciesDrosophila melanogaster (female)
RegionCentral brain (~50%)
Volume250 × 250 × 250 μm
Cells~25,000 neurons
Synapses~20 million
Data size~100 TB
Resolution8 nm isotropic
ModalityFIB-SEM
AccessOpen; neuPrint query interface
StatusReleased

The hemibrain covers roughly half the central brain of an adult female fly at 8 nm isotropic resolution — the highest-quality large fly volume, because FIB-SEM mills rather than sections and so avoids the alignment problems of serial sectioning. It contains about 25,000 neurons and 20 million synapses, and the neuPrint interface made connectivity queryable without downloading anything.

What it is good for. Learning to query a connectome. neuPrint’s Cypher interface is the gentlest on-ramp to thinking of neurons as a graph, and the circuits it resolved — mushroom body learning, central complex navigation, circadian — are the worked examples the field teaches from.

What it does not support. Whole-brain claims: the optic lobes and much of the periphery are absent, so any neuron whose arbor leaves the volume is truncated. Comparisons with FlyWire need care, since the two use different synapse-detection pipelines and different criteria for what counts as a connection.

Where these numbers come from. Scheffer et al. (2020), eLife 10.7554/eLife.57443; neuPrint. DOI 10.7554/eLife.57443.

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