Deck Spec: 07 Glia

Session

• Duration: 70 + 20 min
• Outcome: glia recognition checklist and adjudication notes
• Primary source: course/source-ingest/microns-proofreading-glia-111821.pptx.md
• Figure source: course/units/figures/07-glia-selected-v1.md

Slide production blocks

1. Title and rationale
   • Key message: glia labeling is core to connectome validity, not optional cleanup.
   • Figure: FIG-RIV-GLIA-S01-01
2. Astrocyte morphology cues
   • Key message: astrocyte context depends on process shape plus neighborhood.
   • Figure: FIG-RIV-GLIA-S03-01
3. Microglia recognition cues
   • Key message: surveillance morphology requires multi-slice confirmation.
   • Figure: FIG-RIV-GLIA-S09-01
4. Oligodendrocyte and myelin context
   • Key message: myelin-associated cues prevent neuron/glia boundary errors.
   • Figure: FIG-RIV-GLIA-S15-01
5. Myelin-producing glia context panel
   • Key message: local myelin architecture improves class confidence.
   • Figure: FIG-RIV-GLIA-S16-01
6. Ambiguity classes and escalation rules
   • Key message: classify uncertainty type before adjudication.
   • Figure: reuse FIG-RIV-GLIA-S09-01
7. QC metrics for glia labeling
   • Key message: track glia-vs-neuron boundary error rates by region.
   • Figure: no new figure required (metrics table).
8. Team operations
   • Key message: second-pass review queue is mandatory for low-confidence calls.
9. Failure modes
   • Key message: over-calling microglia and under-reviewing glia cause graph distortions.
10. Activity instructions
    • Key message: classify two ambiguous regions with justification.
    • Figure: FIG-RIV-GLIA-S03-01
11. Debrief
    • Key message: glia corrections should be included in high-impact proofreading triage.
12. Bridge to Unit 08
    • Key message: segmentation/proofreading integrates all prior ambiguity protocols.

Assessment

• Prompt: provide two glia calls with evidence and uncertainty label.
• Rubric: class discrimination quality and justification strength.