Deck Spec: 05 Neuronal Ultrastructure

Session

• Duration: 75 + 20 min
• Outcome: cue-based annotation sheet with confidence labels
• Primary source: course/source-ingest/microns-proofreading-neuronal-ultrastructure-111821.pptx.md
• Figure source: course/units/figures/05-neuronal-ultrastructure-selected-v1.md

Slide production blocks

1. Title and task framing
   • Key message: ultrastructure interpretation is a reproducible decision process, not visual intuition.
   • Figure: FIG-RIV-ULTRA-S04-01
   • Instructor note: define evidence tiers up front.
2. Compartment map refresher
   • Key message: process identity begins with compartment-level anatomy.
   • Figure: FIG-RIV-ULTRA-S08-01
   • Instructor note: ask learners to label soma/shaft/spine candidates live.
3. Synapse-identification cues
   • Key message: vesicle clusters + active zone + PSD context are evaluated together.
   • Figure: FIG-RIV-ULTRA-S09-01
   • Instructor note: require at least two converging cues before a hard call.
4. Organelle context for annotation
   • Key message: mitochondria, ER, and microtubules reduce ambiguity.
   • Figure: FIG-RIV-ULTRA-S10-01
   • Instructor note: show one false-positive cue and how context corrects it.
5. Comparative ultrastructure
   • Key message: similar-looking profiles diverge when local neighborhood is included.
   • Figure: FIG-RIV-ULTRA-S14-01
   • Instructor note: emphasize multi-slice review requirement.
6. Ambiguity handling protocol
   • Key message: uncertainty labels preserve scientific validity.
   • Figure: FIG-RIV-ULTRA-S20-01
   • Instructor note: model a provisional label workflow.
7. Advanced case review
   • Key message: difficult regions need explicit decision logs.
   • Figure: FIG-RIV-ULTRA-S24-01
   • Instructor note: compare two annotator interpretations and adjudicate.
8. Synthesis panel
   • Key message: cue triangulation scales to complex scenes when procedure is consistent.
   • Figure: FIG-RIV-ULTRA-S30-01
   • Instructor note: close with decision checklist learners can reuse.
9. Failure modes and anti-patterns
   • Key message: single-slice calls, single-cue dependence, and forced certainty drive major errors.
   • Figure: reuse FIG-RIV-ULTRA-S20-01
10. QC metrics and auditability
    • Key message: inter-rater agreement and unresolved-case rates are operational quality indicators.
    • Figure: no new figure required (table slide).
11. Activity instructions
    • Key message: annotate one panel with confidence + alternate hypothesis.
    • Figure: FIG-RIV-ULTRA-S24-01
12. Debrief and bridge to Unit 06
    • Key message: process-type classification extends this cue logic.

Assessment

• Prompt: annotate one panel and justify decisions with three independent cues.
• Rubric: cue quality, coherence, uncertainty handling.