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Deck Spec: 03 EM Prep and Imaging

Session

Slide production blocks

  1. Title and scope
    • Key message: acquisition quality sets the upper bound for reconstruction quality.
    • Figure: FIG-SRC-MODULE12_LESSON3-S02-01
  2. Imaging context and motivation
    • Key message: high-resolution imaging is necessary but not sufficient for valid inference.
    • Figure: FIG-SRC-MODULE12_LESSON3-S04-01
  3. Why connectomics needs disciplined acquisition
    • Key message: acquisition artifacts propagate to downstream segmentation errors.
    • Figure: FIG-SRC-MODULE12_LESSON3-S06-01
  4. High-throughput sectioning context
    • Key message: throughput choices create distinct artifact profiles.
    • Figure: FIG-SRC-MODULE12_LESSON3-S08-01
  5. Imaging pipeline handoff
    • Key message: each handoff requires metadata and QC checkpoints.
    • Figure: FIG-SRC-MODULE12_LESSON3-S10-01
  6. Volume and voxel interpretation
    • Key message: resolution and voxel geometry define what can be interpreted.
    • Figure: FIG-SRC-MODULE13_LESSON2-S02-01
  7. Reconstruction/tomography bridge
    • Key message: imaging choices affect reconstruction complexity.
    • Figure: FIG-SRC-MODULE13_LESSON2-S04-01
  8. Manual vs automated workflow context
    • Key message: automation gains require higher QA discipline, not less.
    • Figure: FIG-SRC-MODULE13_LESSON2-S08-01
  9. Quality comparison panel
    • Key message: artifact classes should be tracked with quantitative triggers.
    • Figure: FIG-SRC-MODULE13_LESSON2-S09-01
  10. Risk register template
    • Key message: list artifact, detection metric, mitigation, and escalation trigger.
  11. Activity instructions
    • Key message: produce one acquisition QA gate plan.
  12. Debrief and bridge to Unit 04
    • Key message: infrastructure design must absorb acquisition variability.

Assessment