Module 09: Neuron Morphology and Skeletonization

Teaching Deck

Learning Objectives

• Generate skeleton representations from reconstructed neurites
• Compute core morphology descriptors
• Relate morphology metrics to biological interpretation
• Report morphology uncertainty and classification limits

Session Outcomes

• Learners can complete the module capability target.
• Learners can produce one evidence-backed artifact.
• Learners can state one limitation or uncertainty.

Agenda (60 min)

• 0-10 min: Frame and model
• 10-35 min: Guided practice
• 35-50 min: Debrief and misconception correction
• 50-60 min: Competency check + exit ticket

Capability Target

Produce a skeleton-based morphology summary with at least three descriptors and one explicit limitation.

Concept Focus

Core Workflow

• Build skeleton from reconstruction.
• Compute descriptors (path length, branching, etc.).
• Compare against reference patterns.
• Report interpretation confidence.

60-Minute Run-of-Show

• 00:00-10: morphology overview.
• 10:00-24: skeleton extraction demo.
• 24:00-38: descriptor calculation.
• 38:00-50: interpretation and caveats.
• 50:00-60: competency check.

Misconceptions to Watch

• Surface and correct one likely misconception during debrief.

Studio Activity

Extract one skeleton and submit a morphology descriptor table with interpretation notes.

Activity Output Checklist

• Evidence-linked artifact submitted.
• At least one limitation or uncertainty stated.
• Revision point captured from feedback.

Assessment Rubric

• Minimum: valid skeleton and descriptor set.
• Strong: robust interpretation with uncertainty framing.
• Failure: descriptor list without biological context.

Exit Ticket

Explain one morphology feature that could be confounded by reconstruction quality.

References (Instructor)

• Use module references listed on the module page.

Teaching Materials

• Module page: /modules/module09/
• Slide page: /modules/slides/module09/
• Worksheet: /assets/worksheets/module09/module09-activity.md